Hydrogen sulfide down-regulates the expression and release of osteoprotegerin (OPG) by vascular endothelial cells

Autori: Erika Rimondi,Maria Grazia di Iasio, Arianna Gonelli,Claudio Celeghini, Paola Secchiero,Giorgio Zauli E. Rimondi, M. G. di Iasio, A. Gonelli, P. Secchiero
Titolo: Hydrogen sulfide down-regulates the expression and release of osteoprotegerin (OPG) by vascular endothelial cells
Descrizione: Osteoprotegerin (OPG) is a soluble member of the tumor necrosis factor (TNF) receptor family, initially characterized for its ability to inhibit the receptor activator of NfkB ligand (RANKL)-stimulated formation of osteoclasts. OPG also interacts with TNF-related apoptosis inducing ligand (TRAIL), a different member of the TNF super-family whose extracellular domain shares a 35% homology with RANKL. Several studies have demonstrated that OPG is elevated in the serum/plasma of patients affected by different types of cancer, but the potential role of OPG with respect to cancer development/progression is unknown. Among different potential cellular sources of circulating OPG, endothelial cells represent a major source of OPG under basal conditions as well as in response to inflammatory stimuli. Hydrogen sulfide (H2S)-known for decades as a toxic gas—is endogenously generated from cysteine, in reactions catalyzed by cystathionineß-synthase(CBS) and cystathionine y-lyase (CSE). Mounting data on endogenously generated H2S have now included this gas in the family of gasotransmitters, together with nitric oxide (NO) and carbon monoxide (CO), and its effects are intensively investigated both at the cellular and molecular level. On these bases, the aim of the present study was to investigate in vitro the effect of H2S on the expression and release of OPG by human vascular endothelial cells in the absence or presence of the pro-inflammatory cytokine TNF-a
Istituto promotore: Department of Morphology and Embryology and LTTA Centre, University of Ferrara, Ferrara, Italy C. Celeghini,* G. Zauli – Institute for Maternal and Child Health, IRCCS “Burlo Garofolo”, Trieste, Italy G. Zauli Department of Morphology and Embryology, Human Anatomy Section, University of Ferrara
Rivista: Invest New Drugs DOI 10.1007/S10637-011-9675-8 © Springer Science+Business Media, LLC 2011
Sezione: farmacologia – ricerca tratta dal sito Fondazione Forst